Anti-Fatigue Effects of Pine Pollen Flavonoids

Study on Anti-fatigue Effects of Total Flavonoids from Masson Pine Pollen on Mice

Author: Wu Jingjing (2010), Chinese Journal of Food Science

Flavonoids from Masson pine pollen have been studied for their potential health benefits, including their anti-fatigue properties. This study by Wu Jingjing (2010) investigates the effectiveness of these flavonoids in reducing fatigue in mice through a series of controlled experiments.


To evaluate the anti-fatigue effects of total flavonoids extracted from Masson pine pollen by measuring the swimming endurance and biochemical markers in mice.


  • Animal Model: Mice were divided into different groups and administered varying doses of pine pollen flavonoids.
  • Fatigue Assessment: The mice were subjected to a weight-loaded swimming test to measure endurance.
  • Biochemical Analysis: Post-exercise levels of blood lactic acid, urea nitrogen, and hepatic glycogen were measured to evaluate the impact of pine pollen flavonoids on fatigue and energy metabolism.


  • Swimming Time: Mice administered with pine pollen flavonoids showed significantly longer swimming times compared to the control group, indicating enhanced endurance.
  • Biochemical Parameters:
    • Blood Lactic Acid: Flavonoid-treated mice had lower levels of blood lactic acid post-exercise, suggesting reduced muscle fatigue.
    • Urea Nitrogen: A reduction in urea nitrogen levels was observed in the flavonoid-treated groups, indicating improved metabolic recovery.
    • Hepatic Glycogen: Higher hepatic glycogen content was noted in the flavonoid-treated mice, reflecting better energy storage and utilization.

Research Significance

Understanding the anti-fatigue effects of pine pollen flavonoids is important for developing natural supplements that can enhance physical performance and reduce fatigue. This study provides scientific evidence supporting the use of pine pollen flavonoids as a potential supplement for athletes and individuals seeking to improve endurance and recovery.


The study supports the hypothesis that total flavonoids from Masson pine pollen can significantly reduce fatigue and enhance physical performance. These effects are attributed to the antioxidant properties of flavonoids, which help mitigate oxidative stress and improve energy metabolism in mice.


Wu, J. (2010). Study on Anti-fatigue Effects of Total Flavonoids from Masson Pine Pollen on Mice. Chinese Journal of Food Science.