The Immune Modulating Properties of Pine Pollen
Paper Title: Influence Of Pine Pollen On Immune Modulating Functions. Zhang Tun, Jianping Zhu. Chinese Journal Of Hospital Pharmacy, 2006(5): 638-639
Zhang Tun and Jianping Zhu conducted an observational research project on the influence of Pine Pollen on the immune modulating function in mice.
Random group the mice into negative control group(purified water), low-dosage, medium-dosage and high-dosage groups, and feed each group 0.25g, 0.5g and 1.5g/kg (body weight) with cell-broken Pine Pollen powder which is equivalent to 5 times, 10 times and 30 times of human clinical dosage. 30 days later, serum hemolysin measurement, mice peritoneal macrophages phagocytosis ability test on chicken erythrocyte, dinitrofluorobenzene-induced delayed type hypersensitivity test (DTH), NK cell activity test, mice antibody formation(Hemolytic plaque number), thymus index and spleen index test were conducted.
Influence of Pine Pollen on the Immune Modulation Function in Mice
|Group||Antibody Titre Level||Phagocytic Rate (%)||Phagocytic Index||Swelling Level (mg)||NK Cell Activity (%)||Thymus Index (%)||Spleen Index (%)||Hemolytic Plaque Number (*1000/Total Splenectomy)||OD Value + Control A||OD Value w/o Control A||Difference Value|
|Negative Control Group||66.0 ± 18.5||11 ± 2.0||0.17 ± 0.03||19.6 ± 3.1||27.4 ± 3.9||0.26 ± 0.04||0.64 ± 0.07||9.7 ± 2.2||1.388 ± 0.027||1.304 ± 0.020||0.084 ± 0.009|
|Low Dosage Group||68.6 ± 24.2||11 ± 1.6||0.17 ± 0.03||17.6 ± 5.4||29.7+4.8||0.27 ± 0.05||0.66 ± 0.13||9.7 ± 2.9||1.405 ± 0.033||1.314 ± 0.023||0.091 ± 0.014|
|Medium Dosage Group||101.2 ± 25.1 (a)||14+1.9 (a)||0.23 ± 0.04 (a)||22.4+3.5||33.0 ± 49 (a)||0.26 ± 0.04||0.68 ± 0.16||14.4 ± 2.2 (a)||1.433 ± 0.032 (a)||1.312 ± 0.020||0.121 ± 0.015 (a)|
The antibody titre level, phagocytic rate, phagocytic index, swelling level, hemolytic plaque number, NK cell activity, and the left and right ear difference value of dinitrofluorobenzene-induced delayed type hypersensitivity show a significant increase for the medium-dosage and high-dosage groups compared with the negative control group, while the Thymus index and Spleen index show no great change.
The above experiment shows that cell cracked Pine Pollen can greatly increase the cellular immune function and cytophagy function, and that this change may be attributed to the nutrition contained in Pine Pollen.
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